19th P4EU Meeting

May 22, 2023, 9:00 AM → May 23, 2023, 6:00 PM Europe/Rome

Trieste, Italy

Paola Storici (Elettra Sincrotrone Trieste SCpA)

19th P4EU Meeting

22 - 23 of May 2023
AREA Science Park - Padriciano, 99 - Trieste

Elettra Sincrotrone Trieste and International Centre For Genetic Engineering and Biotechnology (ICGEB) are proud to host the 19th Annual Meeting of the Protein Production and Purification Partnership in Europe.

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The Partnership groups more than 200 public and private facilities all over Europe with the mission to facilitate communication and exchange of information. 

The annual meeting brings together 50+ experts from research organizations, universities and the private sector. 

This high-level scientific event is centred around new expression and purification technologies, protein characterization expert panels, process optimization, quality control and new applications. 

How to reach us.pdf

Monday, May 22

WORKSHOP:: Methods for protein characterization and quality control

1 Welcome and Workshop openings

2 Understanding biomolecular behaviour with mass photometry

Mass photometry, an analytical technology for biomolecular characterization, measures the mass of single particles in solution. The TwoMP is an easy-to-use instrument that can measure the mass and relative abundance of particles between 30 kDa and 5 MDa, with little sample. With mass photometry is possible to study antibody-antigen interactions, quantify small-molecule induced changes to complex formation, assess sample purity and more.

Speaker: Perla Vega Dominguez (Refeyn - sponsor presentation)

3 A closer insight into intermolecular interactions characterization: Hands on Creoptix Wave

10:40 AM Coffe Break

4 Methods applied at Elettra for protein fold and binding analysis (FTIR, AFM, Raman)

Presentations from Elettra beamline scientists on biophysical methods applied using FTIR, UV-Raman spectroscopy and AFM to detect protein fold and protein-ligand bindings.
• 11:00 Lisa Vaccari - Infrared spectroscopy and nanoscopy: new frontiers in protein studies (SISSI beamline @ Elettra)
• 11:20 Barbara Rossi - UV Resonance Raman for characterization of proteins (IUVIS beamline @ Elettra)
• 11:40 Loredana Casalis - AFM nanotechnology (NanoLab @ Elettra)
• 12:00 Annie Heroux - One step further on the protein characterization process: XRD2 dedicated MX beamline at Elettra (XRD2 beamline @ Elettra)

5 Alfatest, sponsor presentation

Roberto Santoliquido - Triple detector Size Exclusion Chromatography for biomolecules characterization (Alfatest, sponsor presentation)

Speaker: Roberto Santoliquido (Alfatest)

6 General discussion and Closure

1:00 PM Lunch

19th P4EU Annual Meeting

• P4EU openings - Welcome from Organizers
• Presentation of the AREA science Park and PNRR project
• Talks from P4EU members on protein for integrated structural biology (focus on Cryo-EM and new developments)
• New facility presentation

7:30 PM Social dinner

TBD

Tuesday, May 23

Morning Session

• Presentation on EEN and case studies (Lara Dipace)
• Hot topics in P4EU, new challenges and techniques
• Talks from P4EU members on non-conventional expression systems

1:00 PM Lunch

General discussion P4EU projects: benchmarking activities, COST actions

3:25 PM Visits to AREA, ICGEB and Elettra laboratories